Transcriptome sequencing of Microcystis aeruginosa - SRA

SRX25882077: Transcriptome sequencing of Microcystis aeruginosa
1 ILLUMINA (Illumina NovaSeq 6000) run: 10.2M spots, 3.1G bases, 1.5Gb downloads

Design: RNA-seq strand-specific libraries were prepared following the TruSeq RNA sample preparation Kit from Illumina (San Diego, CA, USA), using 5 g of total RNA. Briefly, rRNA removal was performed using the RiboZero rRNA removal kit (Epicenter, WI, USA), and the RNA was then fragmented using fragmentation buffer. Subsequently, cDNA synthesis, end repair, A-base addition, and ligation of the Illumina-indexed adaptors were carried out according to Illuminas protocol. The libraries were size selected for cDNA target fragments of 200 300 bp on 2% Low Range Ultra Agarose, followed by PCR amplification using Phusion DNA polymerase (NEB) for 15 PCR cycles. After quantification using TBS380, the paired-end libraries were sequenced using the Illumina NovaSeq 6000 sequencing platform (150bp*2, Shanghai BIOZERON Co., Ltd).

Submitted by: Tongji University

Study: Transcriptome sequencing of Microcystis aeruginosa

PRJNA1153622 • SRP529255 • All experiments • All runs

show Abstracthide Abstract

The sequencing data is used to study the effect of antibiotics on Microcystis aeruginosa (strain PCC 7806) with different growth rates.

Sample: 48 h after adding; Growth rate: 0.20; Parallel sample 1

SAMN43400272 • SRS22485915 • All experiments • All runs

Organism: Microcystis aeruginosa PCC 7806

Library:

Name: MA_D0.20-A_1_R1

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq